APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
rat brain , Mouse brain , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950529
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13
Cellular Localization
Mitochondrion matrix
Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110089 WB Image
HSP60 antibody detects HSPD1 protein by western blot analysis. A. 50 μg rat brain lysate/extract 7.5% SDS-PAGE HSP60 antibody (GTX110089) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
Sample (20 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX110089 diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 ICC/IF Image
HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 IHC-P Image
HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110089 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110089 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp60A is the homolog of mammalian Hsp60. (Gene ID: 32045/ UniProt: O02649)
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REFERENCE
REVIEW
HSP60 antibody
Cat. No. GTX110089
Rating
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( Average 4.9 based on 9 users reviews)
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Application
Western Blot(WB)
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( Average 4.9 based on 9 users reviews)
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Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 27089984
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 27089984
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Brain in 2014.
PMID: 24727571
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal J Biomed Sci in 2013.
PMID: 24359494
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2018.
PMID: 29950685
Date :
Anonymous submitted on 18-Apr-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells, H1975 cells
Amount used :
30 μg
Blocking :
5% BSA, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 08-Jan-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
PANC1 cells
Amount used :
25 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 23-Aug-2016
Application Tested :
WB
Sample Species :
Hm
Sample :
CHO DP 12 clone#1933 cells
Amount used :
40μg
Blocking :
3% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 13-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
W12 cells
Blocking :
1% skim milk in PBST, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
Lane Description :
Lane 1: W12 total cell lysate, Lane 2: W12 cytosol extract, Lane 3: W12 mitochondrial extract