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HSP60 antibody

Orthogonal
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089

Cat. No. GTX110089

Reference 23
9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila, Hamster
Package
100 μl ($399),
25 μl ($169)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

rat brain , Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950529

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

HSP60 antibody detects HSPD1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
HSP60 antibody (GTX110089) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110089 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110089

GTX110089 ICC/IF Image

HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HSP60 stained by HSP60 antibody (GTX110089) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp60A is the homolog of mammalian Hsp60. (Gene ID: 32045/ UniProt: O02649)

REFERENCE

Application Reference

Chen KC et al. J Clin Med 2021; 11 (1) Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics.
Application : WB
Reactivity : Rat
Lin TY et al. Phytomedicine 2021; 80 : 153384 Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells.
Application : WB
Reactivity : Mouse
Hari Prasad Osuru et al. Front Med (Lausanne) 2020; 7 : 581082 Anesthesia-Sepsis-Associated Alterations in Liver Gene Expression Profiles and Mitochondrial Oxidative Phosphorylation Complexes.
Application : WB
Reactivity : Rat
P?rez-Rodriguez S et al. PLoS One 2020; 15 (8) : e0237930 Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.
Application : WB
Reactivity : Hamster
Naeem S et al. Toxicol Lett 2020; 326 : 1-10 NIX compensates lost role of parkin in cd-induced mitophagy in HeLa cells through phosphorylation.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

HSP60 antibody Cat. No. GTX110089

Rating
( Average 4.9 based on 9 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 9 users reviews)
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27089984
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27089984
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Brain in 2014. PMID: 24727571
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2013. PMID: 24359494
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29950685
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 18-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, H1975 cells
Amount used : 30 μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO DP 12 clone#1933 cells
Amount used : 40μg
Blocking : 3% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Date : Anonymous submitted on 13-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : W12 cells
Blocking : 1% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane 1: W12 total cell lysate, Lane 2: W12 cytosol extract, Lane 3: W12 mitochondrial extract
Show More +
Submit a Review
Application Reference
Chen KC et al. J Clin Med 2021; 11 (1) Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics.
Application : WB
Reactivity : Rat
Lin TY et al. Phytomedicine 2021; 80:153384 Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells.
Application : WB
Reactivity : Mouse
Hari Prasad Osuru et al. Front Med (Lausanne) 2020; 7:581082 Anesthesia-Sepsis-Associated Alterations in Liver Gene Expression Profiles and Mitochondrial Oxidative Phosphorylation Complexes.
Application : WB
Reactivity : Rat
P?rez-Rodriguez S et al. PLoS One 2020; 15 (8):e0237930 Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.
Application : WB
Reactivity : Hamster
Naeem S et al. Toxicol Lett 2020; 326:1-10 NIX compensates lost role of parkin in cd-induced mitophagy in HeLa cells through phosphorylation.
Application : WB
Reactivity : Human
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
Application : WB
Reactivity : Human
Mallach A et al. FASEB J 2019; 33 (6):6957-6961 Post mortem examination of Parkinson's disease brains suggests decline in mitochondrial biomass, reversed by deep brain stimulation of subthalamic nucleus.
Application : IHC-P
Reactivity : Human
Chou CK et al. Int J Mol Sci 2018; 19 (7) Ethyl Acetate Extract of Scindapsus Cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells Through Modulating ER Stress.
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : ICC/IF
Reactivity : Human
De Maio F et al. Cell Microbiol 2018;:e12952 PE_PGRS3 of Mycobacterium tuberculosis is specifically expressed at low phosphate concentration, and its arginine-rich C-terminal domain mediates adhesion and persistence in host tissues when expressed in Mycobacterium smegmatis.
Application : WB
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Wakae K et al. Sci Rep 2018; 8 (1):9745 Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.
Application : WB
Reactivity : Human
Gu X et al. Toxicol Lett 2018; 291:92-100 Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Application : WB
Reactivity : Human
Won SJ et al. J FUNCT FOODS 2016; 24:568-578 A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells.
Application : WB
Reactivity : Human
Niu Z et al. Environ Toxicol Pharmacol 2016; 45:212-221 mitophagy inhibits proliferation by decreasing cyclooxygenase-2 (cox-2) in *-treated hepg2 cells.
Application : ICC/IF
Reactivity : Human
Qi Y et al. Sci Rep 2016; 6:24700 ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
Application : WB, ICC/IF
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : ELISA
Reactivity : Human
Wei X et al. Toxicol Mech Methods 2014;:43124 Cadmium induces mitophagy through ROS-mediated PINK1/Parkin pathway.
Application : WB
Reactivity : Mouse
Pfeffer G et al. Brain 2014; Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.
Application : WB
Reactivity : Human
Chen JY et al. J Biomed Sci 2013; 20:95 quercetin-induced cardioprotection against * cytotoxicity.
Application : WB
Reactivity : Rat
Li JM et al. Environ Mol Mutagen 2013; 54 (6):429-49 Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Application : WB
Reactivity : Human
Reina CP et al. Cell Stress Chaperones 2012; 17 (6):729-42 Basal and stress-induced Hsp70 are modulated by ataxin-3.
Application : WB
Reactivity : Mouse
Zhang A et al. Mol Cell Proteomics 2011; 10 (10):M111.009936 Quantitative proteomic analyses of human cytomegalovirus-induced restructuring of endoplasmic reticulum-mitochondrial contacts at late times of infection.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169