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HSP60 antibody

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089
Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110089
Anti-HSP60 antibody used in Western Blot (WB). GTX110089

Cat No. GTX110089

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat, Hamster
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family D (Hsp60) Member 1,Cpn60,Groel,Hld4,Hsp-60,Hsp60,Hsp65,Hucha60,Spg13,Hspd1

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondrion on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX110089

GTX110089 IHC-P Image

HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
HSP60 antibody (GTX110089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

HSP60 antibody detects HSPD1 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
HSP60 antibody (GTX110089) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Sample (20 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110089 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110089

GTX110089 ICC/IF Image

HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HSP60 antibody used in Western Blot (WB). GTX110089

GTX110089 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody (GTX110089) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

HSP60 antibody Cat No. GTX110089

Rating ( Average 4.8 based on 4 users reviews)
Application
Date : Anonymous submitted on 18-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, H1975 cells
Amount used : 30 μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO DP 12 clone#1933 cells
Amount used : 40μg
Blocking : 3% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 13-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : W12 cells
Blocking : 1% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane 1: W12 total cell lysate, Lane 2: W12 cytosol extract, Lane 3: W12 mitochondrial extract
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169