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HSP60 antibody

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX11540
Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11540
Anti-HSP60 antibody used in Western Blot (WB). GTX11540

Cat. No. GTX11540

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60(503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family D member 1 , Hsp60 , Hspd1-30p

Cellular Localization

Mitochondrion matrix

Background

a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX11540

GTX11540 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX11540 HSP60 antibody.

Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11540

GTX11540 ICC/IF Image

ICC/IF analysis of SMMC cell using GTX11540 HSP60 antibody.

Anti-HSP60 antibody used in Western Blot (WB). GTX11540

GTX11540 WB Image

WB analysis of various samples using GTX11540 HSP60 antibody.
Lane 1 : rat brain tissue lysate
Lane 2 : rat liver tissue lysate
Lane 3 : CEM cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : SMMC cell lysate
Lane 6 : COLO320 cell lysate

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449