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HSP60 antibody

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX32661
Anti-HSP60 antibody used in Immunoprecipitation (IP). GTX32661
Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32661
Anti-HSP60 antibody used in Western Blot (WB). GTX32661

Cat No. GTX32661

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human HSPD1 (NP_002147.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody used in IHC (Paraffin sections) (IHC-P). GTX32661

GTX32661 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32661 HSP60 antibody.
Dilution : 1:100

Anti-HSP60 antibody used in Immunoprecipitation (IP). GTX32661

GTX32661 IP Image

IP analysis of HeLa cell lysate using GTX32661 HSP60 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Anti-HSP60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32661

GTX32661 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32661 HSP60 antibody.
Blue : DAPI

Anti-HSP60 antibody used in Western Blot (WB). GTX32661

GTX32661 WB Image

WB analysis of various samples using GTX32661 HSP60 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329