GeneTex
United States (US)

HSP70 1L antibody [C1C3]

GTX105164_40002_20160414_WB_19011506_520.jpg
GTX105164_WB_19011506_845.jpg
GTX105164_40002_20160310_WB_R_testis_19011506_492.jpg
GTX105164_40002_IFA_19011506_923.jpg
GTX105164_40002_20160310_WB_M_testis_19011506_912.jpg

Cat No. GTX105164

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse testis , rat testis , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP70 1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSP70-1L antibody, HSP70-HOM antibody, HSP70T antibody, hum70t antibody, HSPA1L antibody, heat shock 10kDa protein 1-like antibody, heat shock 70 kDa protein 1L antibody, heat shock 70kD protein-like 1 antibody, heat shock 70 kDa protein 1-Hom antibody, heat shock 70 kDa protein 1-like antibody, heat shock 70kDa protein 1-like antibody

Background

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105164_40002_20160414_WB_19011506_520.jpg

GTX105164 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105164_WB_19011506_845.jpg

GTX105164 WB Image

Sample(30 µg whole cell lysate)
A: H1299
B: HeLa S3 (GTX14654)
C: HepG2 (GTX27900)
D: MOLT4 (GTX27912)
E: Raji (GTX27908)
7.5% SDS PAGE
GTX105164 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105164_40002_20160310_WB_R_testis_19011506_492.jpg

GTX105164 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105164_40002_IFA_19011506_923.jpg

GTX105164 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSP70 1L(GTX105164) antibody at 1:500 dilution.

GTX105164_40002_20160310_WB_M_testis_19011506_912.jpg

GTX105164 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169