APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse testis , rat testis , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP70 1L. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950522
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 1 like , HSP70-1L , HSP70-HOM , HSP70T , hum70t
Background
This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105164 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105164 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105164 WB Image
Sample(30 μg whole cell lysate) A: H1299 B: HeLa S3 (GTX14654) C: HepG2 (GTX27900) D: MOLT4 (GTX27912) E: Raji (GTX27908) 7.5% SDS PAGE GTX105164 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105164 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105164 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSP70 1L(GTX105164) antibody at 1:500 dilution.
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REFERENCE
There are currently no references for HSP70 1L antibody [C1C3] (GTX105164). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP70 1L antibody [C1C3] (GTX105164). Be the first to share your experience with this product.