Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSP70 1L(GTX105164) antibody at 1:500 dilution.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg whole cell lysate)
A: H1299
B: HeLa S3 (GTX14654)
C: HepG2 (GTX27900)
D: MOLT4 (GTX27912)
E: Raji (GTX27908)
7.5% SDS PAGE
GTX105164 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.