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HSP70 1L antibody [N1N3]

Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045
Anti-HSP70 1L antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111045
Anti-HSP70 1L antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111045
Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045
Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045
Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045
Anti-HSP70 1L antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111045

Cat. No. GTX111045

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSP70 1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Molt-4 , mouse testis , rat testis , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP70 1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1 like , HSP70-1L , HSP70-HOM , HSP70T , hum70t

Background

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045

GTX111045 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP70 1L antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111045

GTX111045 ICC/IF Image

HSP70 1L antibody [N1N3] detects HSP70 1L protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:400.
Blue: Hoechst 33342 staining.
Anti-HSP70 1L antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111045

GTX111045 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using HSP70 1L(GTX111045) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045

GTX111045 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045

GTX111045 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP70 1L antibody [N1N3] used in Western Blot (WB). GTX111045

GTX111045 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111045 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP70 1L antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111045

GTX111045 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using HSP70 1L(GTX111045) antibody at 1:500 dilution.

REFERENCE

Application Reference

Huusko JM et al. PLoS Genet 2018; 14 (7) : e1007394 Whole exome sequencing reveals HSPA1L as a genetic risk factor for spontaneous preterm birth.
Application : WB
Reactivity : Human
Peng YJ et al. Sci Rep 2016; 6 : 32444 Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β.
Application : WB
Reactivity : Human
Wu DW et al. Cell Death Dis 2016; 7 (6) : e2290 TC-N19, a novel dual inhibitor of EGFR and cMET, efficiently overcomes EGFR-TKI resistance in non-small-cell lung cancer cells.
Application : WB
Reactivity : Human
Lin TJ et al. Mol Cancer 2015; 14 : 174 Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169