GeneTex
United States (US)

HSP90 beta antibody

GTX101448_39742_20170706_WB_M_19011506_266.jpg
GTX101448_39742_20170706_WB_R_19011506_838.jpg
GTX101448_39742_IHC_19011506_804.jpg
GTX101448_39742_IHC_M_2_19011506_501.jpg
GTX101448_39742_IHC_M_19011506_814.jpg
GTX101448_WB_19011506_144.jpg
GTX101448_39742_IFA_19011506_191.jpg

Cat No. GTX101448

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA-Ag
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ELISA-Ag Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , *RK3E , *4T1 , *SW480

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HSP90 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein 90 Alpha Family Class B Member 1,D6S182,Hsp84,Hsp90B,Hspc2,Hspcb,Hsp90Ab1

Cellular Localization

Cytoplasm , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

DATA IMAGES
GTX101448_39742_20170706_WB_M_19011506_266.jpg

GTX101448 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody (GTX101448) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101448_39742_20170706_WB_R_19011506_838.jpg

GTX101448 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody (GTX101448) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101448_39742_IHC_19011506_804.jpg

GTX101448 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using HSP90 beta(GTX101448) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101448_39742_IHC_M_2_19011506_501.jpg

GTX101448 IHC-P Image

HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
HSP90 beta antibody (GTX101448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101448_39742_IHC_M_19011506_814.jpg

GTX101448 IHC-P Image

HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
HSP90 beta antibody (GTX101448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101448_WB_19011506_144.jpg

GTX101448 WB Image

Sample(30 µg of whole cell lysate)
A:293T
B:A431(GTX27909)
7.5% SDS PAGE
GTX101448 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101448_39742_IFA_19011506_191.jpg

GTX101448 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101448) antibody at 1:500 dilution.

REFERENCE
REVIEW

HSP90 beta antibody Cat No. GTX101448

Rating ( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 5 users reviews)
Date : Anonymous submitted on 27-Feb-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 10 μg
Blocking : 5% ECL Blocking Agent, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: PC-3 cells, Lane 2: Du145 cells
Date : Anonymous submitted on 26-Jul-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : MC3T3-E1 cells
Amount used : 50 μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Date : Anonymous submitted on 24-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells
Amount used : 40 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, overnightHr
Lane Description : Lane1-Control medium, Lane2- Low folate medium, Lane3- High folate medium
Date : Anonymous submitted on 20-May-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : 4T1 cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 31-Jan-2013
Score :
Application Tested : WB
Sample Species : Rat
Sample : RK3E cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:250, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169