GeneTex
United States (US)

HSPA1B antibody

GTX106148_39785_20160414_WB_19011506_572.jpg
GTX106148_39785_20160629_IFA_19011506_438.jpg
GTX106148_39785_20160120_IHC-P_R_19011506_652.jpg
GTX106148_39785_IHC_19011506_670.jpg

Cat No. GTX106148

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP70 1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ54328 antibody, HSP70-1B antibody, HSP70-2 antibody, HSPA1A antibody, HSPA1B antibody, heat shock 70kD protein 1B antibody, heat shock 70 kDa protein 1/2 antibody, HSP70.1/HSP70.2 antibody, heat shock 70 kDa protein 1A/1B antibody, HSP70-1/HSP70-2 antibody, heat shock 70kDa protein 1B antibody

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106148_39785_20160414_WB_19011506_572.jpg

GTX106148 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1B antibody (GTX106148) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106148_39785_20160629_IFA_19011506_438.jpg

GTX106148 ICC/IF Image

HSPA1B antibody detects HSPA1B protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSPA1B protein stained by HSPA1B antibody (GTX106148) diluted at 1:400.
Blue: Hoechst 33342 staining.

GTX106148_39785_20160120_IHC-P_R_19011506_652.jpg

GTX106148 IHC-P Image

HSP70 1B antibody detects HSP70 1B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HSP70 1B antibody (GTX106148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106148_39785_IHC_19011506_670.jpg

GTX106148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human glioma, using HSP70 1B(GTX106148) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169