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HSPA2 antibody [C1C3]

Anti-HSPA2 antibody [C1C3] used in Western Blot (WB). GTX109739
Anti-HSPA2 antibody [C1C3] used in Western Blot (WB). GTX109739

Cat. No. GTX109739

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSPA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037207

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 2 , HSP70-2 , HSP70-3

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Database

Research Area

DATA IMAGES

Anti-HSPA2 antibody [C1C3] used in Western Blot (WB). GTX109739

GTX109739 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
E: Hep G2 (GTX27900)
F: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109739 diluted at 1:1000

Anti-HSPA2 antibody [C1C3] used in Western Blot (WB). GTX109739

GTX109739 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody [C1C3] (GTX109739) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169