APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT116 , MCF-7 , NIH-3T3 , BCL-1 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , PC-12 , Rat-2 , rat brain
Predict Reactivity
Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSPA2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11171898
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 2 , HSP70-2 , HSP70-3
Background
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Database
Research Area
DATA IMAGES
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GTX110079 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
7.5% SDS PAGE
GTX110079 diluted at 1:10000
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GTX110079 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody (GTX110079) diluted at 1:10000.
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GTX110079 WB Image
Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX110079 diluted at 1:10000
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GTX110079 IHC-P Image
HSPA2 antibody detects HSPA2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HSPA2 antibody (GTX110079) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110079 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody (GTX110079) diluted at 1:10000.
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GTX110079 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody (GTX110079) diluted at 1:10000.
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REFERENCE
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