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HSPA4 antibody [N1N3]

Anti-HSPA4 antibody [N1N3] used in Western Blot (WB). GTX112329
Anti-HSPA4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112329
Anti-HSPA4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112329
Anti-HSPA4 antibody [N1N3] used in Western Blot (WB). GTX112329

Cat. No. GTX112329

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSPA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950523

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 4 , APG-2 , HEL-S-5a , HS24/P52 , HSPH2 , RY , hsp70 , hsp70RY

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-HSPA4 antibody [N1N3] used in Western Blot (WB). GTX112329

GTX112329 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX112329 diluted at 1:1000
Anti-HSPA4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112329

GTX112329 IHC-P Image

HSPA4 antibody [N1N3] detects HSPA4 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HSPA4 antibody [N1N3] (GTX112329) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSPA4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112329

GTX112329 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HSP70 4(GTX112329) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSPA4 antibody [N1N3] used in Western Blot (WB). GTX112329

GTX112329 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA4 antibody [N1N3] (GTX112329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169