Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA4 antibody [N3C2], Internal (GTX112607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSPA4(GTX112607) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
5% SDS PAGE
GTX112607 diluted at 1:1000