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HSPA6 antibody [1H3]

Overexpression
Anti-HSPA6 antibody [1H3] used in Flow cytometry (FACS). GTX84333
Anti-HSPA6 antibody [1H3] used in Western Blot (WB). GTX84333
Anti-HSPA6 antibody [1H3] used in Western Blot (WB). GTX84333
Anti-HSPA6 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84333

Cat No. GTX84333

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H3

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($459)
See all HSPA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HSPA6 (NP_002146) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 6 , HSP70B''

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Database

Research Area

DATA IMAGES

Anti-HSPA6 antibody [1H3] used in Flow cytometry (FACS). GTX84333

GTX84333 FACS Image

FACS analysis of HEK293T cells transfected with either HSPA6 plasmid(Red) or empty vector control plasmid(Blue) using GTX84333 HSPA6 antibody [1H3].

Anti-HSPA6 antibody [1H3] used in Western Blot (WB). GTX84333

GTX84333 WB Image

Overexpression

WB analysis of HEK293T cells transfected with HSPA6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84333 HSPA6 antibody [1H3].
Loading : 5 ug per lane

Anti-HSPA6 antibody [1H3] used in Western Blot (WB). GTX84333

GTX84333 WB Image

WB analysis of various cell lines using GTX84333 HSPA6 antibody [1H3].
Loading : 35 ug per lane

Anti-HSPA6 antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84333

GTX84333 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with HSPA6 plasmid using GTX84333 HSPA6 antibody [1H3].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459