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HSPA6 antibody [C1C3]

Orthogonal
Anti-HSPA6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106192
Anti-HSPA6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX106192
Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192
Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

Cat. No. GTX106192

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HSPA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

H1299

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 6 , HSP70B'

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Database

Research Area

DATA IMAGES

Anti-HSPA6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106192

GTX106192 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6(GTX106192) antibody at 1:200 dilution.

Anti-HSPA6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX106192

GTX106192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using HSPA6(GTX106192) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

GTX106192 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA6 antibody [C1C3] (GTX106192) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

GTX106192 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX106192 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169