GeneTex
  • Country / Location Selection

United States (US)

HSPA6 antibody [C1C3]

Anti-HSPA6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106192
Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192
Anti-HSPA6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX106192
Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

Cat No. GTX106192

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950524

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 6 , HSP70B'

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Database

Research Area

DATA IMAGES

Anti-HSPA6 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106192

GTX106192 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6(GTX106192) antibody at 1:200 dilution.

Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

GTX106192 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX106192 diluted at 1:1000

Anti-HSPA6 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX106192

GTX106192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using HSPA6(GTX106192) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSPA6 antibody [C1C3] used in Western Blot (WB). GTX106192

GTX106192 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA6 antibody [C1C3] (GTX106192) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for HSPA6 antibody [C1C3] (GTX106192). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSPA6 antibody [C1C3] (GTX106192). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169