GeneTex
United States (US)

HSPA6 antibody [C1C3]

GTX106192_IFA_19011506_318.jpg
GTX106192_WB_19011506_315.jpg
GTX106192_40030_IHC_19011506_927.jpg

Cat No. GTX106192

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSPA6 antibody, heat shock 70 kDa protein 6 antibody, heat shock 70 kDa protein B' antibody, heat shock 70kD protein 6 (HSP70B') antibody, heat shock 70kDa protein 6 (HSP70B') antibody

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
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Database

Research Area

DATA IMAGES
GTX106192_IFA_19011506_318.jpg

GTX106192 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6(GTX106192) antibody at 1:200 dilution.

GTX106192_WB_19011506_315.jpg

GTX106192 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX106192 diluted at 1:1000

GTX106192_40030_IHC_19011506_927.jpg

GTX106192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using HSPA6(GTX106192) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf