Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX106192 diluted at 1:1000

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA6 antibody [C1C3] (GTX106192) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SG xenograft, using HSPA6(GTX106192) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6(GTX106192) antibody at 1:200 dilution.