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HSPB2 antibody

Anti-HSPB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87453
Anti-HSPB2 antibody used in Western Blot (WB). GTX87453

Cat. No. GTX87453

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all HSPB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human HSPB2 (121-170).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HSP27 , HSPB2 , Hs.78846 , LOH11CR1K , MKBP , heat shock protein family B (small) member 2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-HSPB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87453

GTX87453 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87453 HSPB2 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HSPB2 antibody used in Western Blot (WB). GTX87453

GTX87453 WB Image

WB analysis of HepG2 and HeLa cell lysates using GTX87453 HSPB2 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399