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HSPC142 antibody [GT1251]

Overexpression
Anti-HSPC142 antibody [GT1251] used in Western Blot (WB). GTX629814
Anti-HSPC142 antibody [GT1251] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629814
Anti-HSPC142 antibody [GT1251] used in Western Blot (WB). GTX629814

Cat. No. GTX629814

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1251

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HSPC142 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HSPC142. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRISC and BRCA1 A complex member 1 , C19orf62 , HSPC142 , MERIT40 , NBA1

Cellular Localization

Cytoplasm , Nucleus

Background

Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.

Database

Research Area

DATA IMAGES

Anti-HSPC142 antibody [GT1251] used in Western Blot (WB). GTX629814

GTX629814 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSPC142 antibody [GT1251] (GTX629814) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSPC142 antibody [GT1251] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629814

GTX629814 ICC/IF Image

C19orf62 antibody [GT1251] detects C19orf62 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C19orf62 protein stained by C19orf62 antibody [GT1251] (GTX629814) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HSPC142 antibody [GT1251] used in Western Blot (WB). GTX629814

GTX629814 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSPC142 antibody [GT1251] (GTX629814) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations