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HSV tag antibody [GT223]

Anti-HSV tag antibody [GT223] used in Western Blot (WB). GTX629073
Anti-HSV tag antibody [GT223] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629073

Cat. No. GTX629073

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT223

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all HSV tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

HSV-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Hsv Tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888091

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-HSV tag antibody [GT223] used in Western Blot (WB). GTX629073

GTX629073 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSV tag antibody [GT223] (GTX629073) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSV tag antibody [GT223] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629073

GTX629073 ICC/IF Image

Hsv Tag antibody [GT223] detects Hsv Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hsv Tag stained by Hsv Tag antibody [GT223] (GTX629073) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations