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HTF9C antibody

Anti-HTF9C antibody used in IHC (Paraffin sections) (IHC-P). GTX131442
Anti-HTF9C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131442
Anti-HTF9C antibody used in Western Blot (WB). GTX131442

Cat No. GTX131442

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , A431 , HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HTF9C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tRNA methyltransferase 2 homolog A,HTF9C

Background

The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

DATA IMAGES
Anti-HTF9C antibody used in IHC (Paraffin sections) (IHC-P). GTX131442

GTX131442 IHC-P Image

HTF9C antibody detects HTF9C protein at cytoplasm and nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
HTF9C antibody (GTX131442) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HTF9C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131442

GTX131442 ICC/IF Image

HTF9C antibody detects HTF9C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HTF9C protein stained by HTF9C antibody (GTX131442) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-HTF9C antibody used in Western Blot (WB). GTX131442

GTX131442 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HTF9C antibody (GTX131442) diluted at 1:3500.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169