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HTRA2 antibody [1B3]

Anti-HTRA2 antibody [1B3] used in Western Blot (WB). GTX53729

Cat. No. GTX53729

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B3

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HTRA2 products

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HTRA2 (134-458aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HtrA serine peptidase 2 , MGCA8 , OMI , PARK13 , PRSS25

Background

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-HTRA2 antibody [1B3] used in Western Blot (WB). GTX53729

GTX53729 WB Image

WB analysis of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain lysates using HtrA2/Omi antibody at a dilution of 1:1,000.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399