GeneTex
United States (US)

HTRA2 antibody [1B3]

GTX53729_WB_18121410_882.jpg

Cat No. GTX53729

Host Mouse
Clonality Monoclonal
Clone Name 1B3
Isotype IgG1
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Calculated MW

49 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HTRA2 (134-458aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PARK13 Antibody , HTRA2 Antibody , OMI Antibody , HtrA serine peptidase 2 Antibody , PRSS25 Antibody

Background

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX53729_WB_18121410_882.jpg

GTX53729 WB Image

WB analysis of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain lysates using HtrA2/Omi antibody at a dilution of 1:1,000.

Package List Price ($)
$ 319