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HTRA2 antibody

Anti-HTRA2 antibody used in Western Blot (WB). GTX110703
Anti-HTRA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX110703
Anti-HTRA2 antibody used in Western Blot (WB). GTX110703

Cat. No. GTX110703

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HTRA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa , HepG2 , Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HTRA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950532

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HtrA serine peptidase 2 , MGCA8 , OMI , PARK13 , PRSS25

Cellular Localization

Mitochondrion intermembrane space , Mitochondrion membrane; Single-pass membrane protein (Potential)

Background

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HTRA2 antibody used in Western Blot (WB). GTX110703

GTX110703 WB Image

HTRA2 antibody detects HTRA2 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
HTRA2 antibody (GTX110703) dilution: 1:1000

Anti-HTRA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX110703

GTX110703 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HTRA2(GTX110703) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HTRA2 antibody used in Western Blot (WB). GTX110703

GTX110703 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody (GTX110703) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169