GeneTex
United States (US)

HURP antibody

DLGAP5-antibody-GTX87559-WB-1_18121410_198.jpg

Cat No. GTX87559

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

95 kDa. ( Note )

Specificity/Sensitivity

DLGAP5 Antibody detects endogenous levels of total HURP protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human HURP.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dlg Associated Protein 5,Dlg7,Hurp,Dlgap5

Cellular Localization

Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.

Background

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

Database

Research Area

DATA IMAGES
DLGAP5-antibody-GTX87559-WB-1_18121410_198.jpg

GTX87559 WB Image

Western blot analysis of extracts from A549/293/HuvEc cells, using DLGAP5 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339