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HUS1 antibody

Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745
Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745
Anti-HUS1 antibody used in Western Blot (WB). GTX113745
Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745
Anti-HUS1 antibody used in Western Blot (WB). GTX113745

Cat. No. GTX113745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HUS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

C8D30 , 293T

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HUS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037213

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HUS1 checkpoint clamp component , hHUS1

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745

GTX113745 IHC-P Image

HUS1 antibody detects HUS1 protein at cytosol and nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
HUS1 antibody (GTX113745) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745

GTX113745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft , using HUS1(GTX113745) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HUS1 antibody used in Western Blot (WB). GTX113745

GTX113745 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX113745 diluted at 1:1000

Anti-HUS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113745

GTX113745 IHC-P Image

HUS1 antibody detects HUS1 protein at cytosol and nucleus on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
HUS1 antibody (GTX113745) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HUS1 antibody used in Western Blot (WB). GTX113745

GTX113745 WB Image

HUS1 antibody detects HUS1 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
12 % SDS-PAGE
HUS1 antibody (GTX113745) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169