GeneTex
United States (US)

HUS1 antibody

GTX32662_20180530_ICCIF_1_18121410_860.jpg
GTX32662_20180530_IP_18121410_492.jpg
GTX32662_20180530_ICCIF_18121410_354.jpg
GTX32662_20180530_WB_18121410_486.jpg

Cat No. GTX32662

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hus1 Checkpoint Clamp Component,Hhus1,Hus1

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX32662_20180530_ICCIF_1_18121410_860.jpg

GTX32662 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32662 HUS1 antibody.
Blue : DAPI

GTX32662_20180530_IP_18121410_492.jpg

GTX32662 IP Image

IP analysis of 293T cell lysate using GTX32662 HUS1 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

GTX32662_20180530_ICCIF_18121410_354.jpg

GTX32662 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32662 HUS1 antibody.
Blue : DAPI

GTX32662_20180530_WB_18121410_486.jpg

GTX32662 WB Image

WB analysis of various samples using GTX32662 HUS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329