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HUS1B antibody

Anti-HUS1B antibody used in Western Blot (WB). GTX48745

Cat No. GTX48745

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:5000
IP 1:100
ELISA 1:20000 - 1:100000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 104-117 of human HUS1B protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HUS1 checkpoint clamp component B

Background

The protein encoded by the Hus1B gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to HUS1. Hus1B is expressed strongly in testis, less in spleen, thymus, prostate, colon and leukocytes.

Database

DATA IMAGES

Anti-HUS1B antibody used in Western Blot (WB). GTX48745

GTX48745 WB Image

Western blot using GeneTex's affinity purified anti-Hus1B antibody shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289