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HYAL1 antibody

Anti-HYAL1 antibody used in Western Blot (WB). GTX113747
Anti-HYAL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113747
Anti-HYAL1 antibody used in Western Blot (WB). GTX113747

Cat. No. GTX113747

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HYAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , Rat liver

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HYAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617783

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hyaluronidase 1 , HYAL-1 , LUCA1 , MPS9 , NAT6

Cellular Localization

Secreted , Lysosome

Background

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HYAL1 antibody used in Western Blot (WB). GTX113747

GTX113747 WB Image

HYAL1 antibody detects HYAL1 protein by Western blot analysis.
A. 50 μg Rat liver lysate/extract
10 % SDS-PAGE
HYAL1 antibody (GTX113747) dilution: 1:1000

Anti-HYAL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113747

GTX113747 IHC-P Image

HYAL1 antibody detects HYAL1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
HYAL1 stained by HYAL1 antibody (GTX113747) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HYAL1 antibody used in Western Blot (WB). GTX113747

GTX113747 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
HYAL1 antibody
GTX113747 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169