Wild-type (WT) and HYPB/SETD2 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HYPB/SETD2 antibody (GTX127905) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HYPB/SETD2 antibody (GTX127905) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HYPB/SETD2 antibody detects HYPB/SETD2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HYPB/SETD2 stained by HYPB/SETD2 antibody (GTX127905) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HYPB/SETD2 antibody detects HYPB/SETD2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HYPB/SETD2 stained by HYPB/SETD2 antibody (GTX127905) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

HYPB/SETD2 antibody detects SETD2 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
HYPB/SETD2 antibody (GTX127905) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min