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HYPB/SETD2 antibody

KO/KD
Anti-HYPB/SETD2 antibody used in Western Blot (WB). GTX127905
Anti-HYPB/SETD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127905
Anti-HYPB/SETD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127905
Anti-HYPB/SETD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127905
Anti-HYPB/SETD2 antibody used in Western Blot (WB). GTX127905

Cat. No. GTX127905

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HYPB/SETD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

288 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HYPB/SETD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2811237

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain containing 2, histone lysine methyltransferase , HBP231 , HIF-1 , HIP-1 , HSPC069 , HYPB , KMT3A , LLS , SET2 , p231HBP

Cellular Localization

Nucleus

Background

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HYPB/SETD2 antibody used in Western Blot (WB). GTX127905

GTX127905 WB Image

KO/KD

Wild-type (WT) and HYPB/SETD2 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HYPB/SETD2 antibody (GTX127905) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HYPB/SETD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127905

GTX127905 IHC-P Image

HYPB/SETD2 antibody detects SETD2 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
HYPB/SETD2 antibody (GTX127905) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HYPB/SETD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127905

GTX127905 ICC/IF Image

HYPB/SETD2 antibody detects HYPB/SETD2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HYPB/SETD2 stained by HYPB/SETD2 antibody (GTX127905) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HYPB/SETD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127905

GTX127905 IHC-P Image

HYPB/SETD2 antibody detects HYPB/SETD2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HYPB/SETD2 stained by HYPB/SETD2 antibody (GTX127905) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HYPB/SETD2 antibody used in Western Blot (WB). GTX127905

GTX127905 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HYPB/SETD2 antibody (GTX127905) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Gonz?lez-Rodr?guez P et al. Cell Death Dis 2020; 11 (1) : 69 SETD2 mutation in renal clear cell carcinoma suppress autophagy via regulation of ATG12.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169