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Hamartin / TSC1 antibody

Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12288
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX12288
Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12288

Cat. No. GTX12288

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all Hamartin / TSC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

129.77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

2.5% BSA, 0.45% NaCl, 0.1% Na₂HPO₄

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin(1150-1164aa QLHIMDYNETHHEHS), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasm,Membrane

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]

Database

Research Area

DATA IMAGES

Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12288

GTX12288 IHC-P Image

IHC-P analysis of rat brain tissue using GTX12288 Hamartin / TSC1 antibody.

Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX12288

GTX12288 WB Image

WB analysis of various samples using GTX12288 Hamartin / TSC1 antibody.
Lane 1 : rat skeletal muscle tissue lysate at 50ug
Lane 2 : rat heart tissue lysate at 50ug
Lane 3 : rat brain tissue lysate at 50ug
Lane 4 : rat lung tissue lysate at 50ug
Lane 5 : 293T whole cell lysate at 40ug
Lane 6 : HeLa whole cell lysate at 40ug
Lane 7 : HT1080 whole cell lysate at 40ug
Lane 8 : SMMC whole cell lysate at 40ug
Dilution : 0.5 μg/mL

Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12288

GTX12288 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX12288 Hamartin / TSC1 antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449