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Hamartin / TSC1 antibody

KO/KD
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX130062
Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130062
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX130062

Cat. No. GTX130062

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Hamartin / TSC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Hamartin / TSC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886167

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TSC complex subunit 1 , LAM , TSC

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]

Database

Research Area

DATA IMAGES

Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX130062

GTX130062 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TSC1 antibody (GTX130062) diluted at 1:1000.

Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130062

GTX130062 IHC-P Image

TSC1 antibody detects TSC1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TSC1 antibody (GTX130062) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX130062

GTX130062 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TSC1 antibody (GTX130062) diluted at 1:1000.

REFERENCE

There are currently no references for Hamartin / TSC1 antibody (GTX130062). Be the first to share your publications with this product.
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REVIEW

Hamartin / TSC1 antibody Cat. No. GTX130062

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Hamartin / TSC1 antibody used in Immunoprecipitation (IP). GTX130062
Date : Anonymous submitted on 06-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 WT and TSC1 KO cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 2 μg to 500 ul of IP Lysis Buffer, together with with 30μl of Dynabeads protein A. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX130062
Date : Anonymous submitted on 06-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and TSC1 KO cells
Amount used : 100μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:500, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169