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Hamartin / TSC1 antibody

Overexpression
Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX89366
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX89366
Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX89366

Cat. No. GTX89366

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Hamartin / TSC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 3-6μg/ml

Note :

IHC-P
Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland.
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89366-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_000359.1) only.

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QLHIMDYNETHHEH, from the C Terminus of the protein sequence according to NP_000359.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TSC complex subunit 1 , LAM , TSC

Cellular Localization

Cytoplasm , Membrane

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]

Database

Research Area

DATA IMAGES

Anti-Hamartin / TSC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX89366

GTX89366 IHC-P Image

IHC-P analysis of human uterus using GTX89366 Hamartin / TSC1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX89366

GTX89366 WB Image

WB analysis of human brain lysate using GTX89366 Hamartin / TSC1 antibody.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Hamartin / TSC1 antibody used in Western Blot (WB). GTX89366

GTX89366 WB Image

Overexpression

WB analysis of HEK293 overexpressing human TSC1 (mock transfection in first lane) using GTX89366 Hamartin / TSC1 antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399