GeneTex
United States (US)

Haptoglobin antibody

GTX103894_39925_20170706_WB_H_plasma_19011506_921.jpg
GTX103894_39925_IHC_19011506_105.jpg

Cat No. GTX103894

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:30000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

human plasma

Predict Reactivity

Mouse, Rat, Rabbit, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.59mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Haptoglobin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BP antibody, HP2ALPHA2 antibody, HPA1S antibody, MGC111141 antibody, HP antibody, haptoglobin antibody, "haptoglobin, alpha polypeptide antibody", "haptoglobin, beta polypeptide antibody", binding peptide antibody, haptoglobin alpha(2FS)-beta antibody, haptoglobin alpha(1S)-beta antibody

Cellular Localization

Secreted

Background

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX103894_39925_20170706_WB_H_plasma_19011506_921.jpg

GTX103894 WB Image

Human plasma (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody (GTX103894) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103894_39925_IHC_19011506_105.jpg

GTX103894 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal gastric, using Haptoglobin(GTX103894) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf