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Haptoglobin antibody

Anti-Haptoglobin antibody used in Western Blot (WB). GTX112962
Anti-Haptoglobin antibody used in Western Blot (WB). GTX112962

Cat. No. GTX112962

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Haptoglobin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa , HepG2 , A375 , human plasma

Predict Reactivity

Mouse, Rat, Rabbit, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Haptoglobin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950507

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

haptoglobin , BP , HP2ALPHA2 , HPA1S

Cellular Localization

Secreted

Background

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Haptoglobin antibody used in Western Blot (WB). GTX112962

GTX112962 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody (GTX112962) diluted at 1:5000.

Anti-Haptoglobin antibody used in Western Blot (WB). GTX112962

GTX112962 WB Image

Haptoglobin antibody detects Haptoglobin protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
10% SDS-PAGE
Haptoglobin antibody (GTX112962) dilution: 1:500

REFERENCE

Application Reference

Lee JH et al. EBioMedicine 2022; 77 : 103903 Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus.
Application : IHC-P
Yang C.S. et al. Mol Biosyst 2017; (Epub) Proteomic Analysis of Prognostic Plasma Biomarkers in Peripheral Arterial Occlusive Disease.
Application : WB
Reactivity : Human
Lin ST et al. Mol Biosyst 2013; 9 (3) : 447-56 redox-proteomic analysis of *-induced altered thiol activity in cardiomyocytes.
Application : ELISA
Reactivity : Human
Lu CH et al. Arch Biochem Biophys 2013; 529 (2) : 146-56 Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Application : ELISA
Reactivity : Human
Chen CC et al. J Proteomics 2012; 75 (12) : 3760-77 Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169