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Hec1 antibody [9G3.23]

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

Cat No. GTX70268

Host Mouse
Clonality Monoclonal
Clone Name 9G3.23
Isotype IgG2a
Application WB, ICC/IF, IHC-Fr, FACS, IP, PLA
Reactivity Human, Hamster, Kangaroo rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ndc80, Kinetochore Complex Component,Hec,Hec1,Hshec1,Kntc2,Tid3,Hsndc80,Ndc80

Cellular Localization

Nuclear

Background

Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.

Database

Research Area

DATA IMAGES
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Confocal immunofluorescence staining (Olympus FV10i) of HEC1, a kinetochore outer layer marker. U2OS cells were fixed by 4% PFA and costained with Hec1 9G3.23 AB (Red; cat# GTX70268; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Hec1 antibody [9G3.23] Cat No. GTX70268

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : CHO cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : 4% PFA in Phem buffer, 37°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton, 37°C, 5Hr
Primary Antibody : 1:250, 37°C, 1Hr
Figure Note : The image shows a CHO cell in prometaphase stained with Hec1 antibody(GTX70268) (green) and DNA (blue). The scale bar is 5 μM.
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 3Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319