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Hec1 antibody [9G3.23]

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

Cat No. GTX70268

Host

Mouse

Clonality

Monoclonal

Clone Name

9G3.23

Isotype

IgG2a

Application

WB, ICC/IF, IHC-Fr, FACS, IP, PLA

Reactivity

Human, Mouse, Hamster, Kangaroo rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_371632

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Confocal immunofluorescence staining (Olympus FV10i) of HEC1, a kinetochore outer layer marker. U2OS cells were fixed by 4% PFA and costained with Hec1 9G3.23 AB (Red; cat# GTX70268; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000.

REFERENCE

REVIEW

Hec1 antibody [9G3.23] Cat No. GTX70268

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32001712
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25329824
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32001712
Date : Anonymous submitted on 31-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : CHO cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : 4% PFA in Phem buffer, 37°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton, 37°C, 5Hr
Primary Antibody : 1:250, 37°C, 1Hr
Figure Note : The image shows a CHO cell in prometaphase stained with Hec1 antibody(GTX70268) (green) and DNA (blue). The scale bar is 5 μM.
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 3Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329