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Hec1 antibody [9G3.23]

Orthogonal
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

Cat. No. GTX70268

Reference 81
11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9G3.23

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, PLA

Reactivity

 Human, Mouse, Hamster, Kangaroo rat
Package
100 μl ($399)
See all Hec1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_371632

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Orthogonal

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Jihee Hong et al. J Ginseng Res 2022; 46 (3) : 481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
Application : ICC/IF
April L Solon et al. Sci Adv 2021; 7 (47) : eabj9812 Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.
Application : ICC/IF
Reactivity : Human
Steblyanko Y et al. EMBO J 2020; 39 (23) : e105432 Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.
Broad AJ et al. J Cell Biol 2020; 219 (3) Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.
Liang C et al. J Cell Biol 2020; 219 (2) Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation.
Show More +
Submit a Reference

REVIEW

Hec1 antibody [9G3.23] Cat. No. GTX70268

Rating
( Average 5 based on 11 users reviews)
Application
Western Blot(WB)
( Average 5 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 6 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 29100415
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biol Open in 2015.PMID: 26685311
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27764815
Anti-Hec1 antibody [9G3.23] used in IHC (Frozen sections) (IHC-Fr). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2010.PMID: 21187932
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26620470
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biol Open in 2015.PMID: 26685311
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 32001712
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25329824
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 32001712
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 31-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : CHO cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : 4% PFA in Phem buffer, 37°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton, 37°C, 5Hr
Primary Antibody : 1:250, 37°C, 1Hr
Figure Note : The image shows a CHO cell in prometaphase stained with Hec1 antibody(GTX70268) (green) and DNA (blue). The scale bar is 5 μM.
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 3Hr
Show More +
Submit a Review
Application Reference
Jihee Hong et al. J Ginseng Res 2022; 46 (3):481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
Application : ICC/IF
April L Solon et al. Sci Adv 2021; 7 (47):eabj9812 Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.
Application : ICC/IF
Reactivity : Human
Steblyanko Y et al. EMBO J 2020; 39 (23):e105432 Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.
Broad AJ et al. J Cell Biol 2020; 219 (3) Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.
Liang C et al. J Cell Biol 2020; 219 (2) Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation.
Yoshida S et al. Nat Commun 2020; 11 (1):2652 Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.
Reactivity : Mouse
Kim S et al. Nat Commun 2020; 11 (1):612 PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation.
Application : ICC/IF
Reactivity : Human
Chuang SH et al. Eur J Med Chem 2020; 191:112118 Discovery of T-1101 tosylate as a first-in-class clinical candidate for Hec1/Nek2 inhibition in cancer therapy.
Application : WB
Reactivity : Human
Malaby HLH et al. J Cell Biol 2019; 218 (4):1218-1234 Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15.
Li W et al. Sci Adv 2019; 5 (3):eaau7566 A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : WB
Asai Y et al. J Cell Biol 2019; 218 (10):3223-3236 Aurora B kinase activity is regulated by SET/TAF1 on Sgo2 at the inner centromere.
Application : WB
Reactivity : Human
Kim S et al. Toxicol In Vitro 2019; 59:115-125 Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
Application : ICC/IF
Reactivity : Human
Hayward D et al. J Cell Biol 2019; (Epub) CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.
Application : ICC/IF
Reactivity : Human
Heidi LH Malaby et al. Life Sci Alliance 2019; 2 (1):e201800169 KIF18A's neck linker permits navigation of microtubule-bound obstacles within the mitotic spindle.
Application : ICC/IF
Reactivity : Human
Alfonso-P?rez T et al. J Cell Biol 2019; (Epub) MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling.
Ferrara M et al. Oncogene 2018; 37 (2):231-240 Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization.
Nye J et al. PLoS One 2018; 13 (10):e0205948 HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX.
Application : WB
Reactivity : Human
Hellmuth S et al. EMBO J 2018; 37 (22) Local activation of mammalian separase in interphase promotes double-strand break repair and prevents oncogenic transformation.
Application : ICC/IF
Reactivity : Human
Janssen LME et al. Curr Biol 2018; (Epub) Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores.
Application : ICC/IF
Reactivity : Human
Petsalaki E et al. Chromosoma 2018; (Epub) Chmp4c is required for stable kinetochore-microtubule attachments.
Application : ICC/IF
Reactivity : Human
Heidi Malaby et al. bioRxiv 2018; (Epub) Kinesin Binding Protein (KBP) buffers the activity of Kif18A and Kif15 in mitosis to ensure accurate chromosome segregation.
Application : ICC/IF
Reactivity : Human
Wolf PG et al. J Cell Sci 2018; (Epub) Studying meiotic cohesin in somatic cells reveals that Rec8-cohesin requires Stag3 and is regulated by Wapl and Sororin.
Application : ICC/IF
Reactivity : Human
Petsalaki E et al. J Cell Biol 2018; (Epub) The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
Application : ICC/IF
Reactivity : Human
Shirnekhi HK et al. Mol Biol Cell 2017; 28 (15):2035-2041 Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Application : ICC/IF
Reactivity : Human
Lee M et al. Oncotarget 2017; 8 (44):77649-77672 The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1.
Application : WB
Reactivity : Human
Park JE et al. Toxicol Lett 2017; 281:110-118 The tobacco carcinogen NNK disturbs mitotic chromosome alignment by interrupting p53 targeting to the centrosome.
Application : ICC/IF
Reactivity : Human
Takagi M et al. bioRxiv 2017;:Epub ahead of print Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.
Application : ICC/IF
Reactivity : Human
Niikura Y et al. Cell Cycle 2017; 16 (18):1683-1694 SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Application : ICC/IF
Reactivity : Human
Janczyk P? et al. Dev Cell 2017; 41 (4):438-449.e4 Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores.
Application : ICC/IF, PLA
Reactivity : Human
Brouwers N et al. PLoS One 2017; 12 (4):e0174819 Role of Kif15 and its novel mitotic partner KBP in K-fiber dynamics and chromosome alignment.
Application : ICC/IF
Reactivity : Human
Huis In 't Veld PJ et al. Elife 2016; 5 Molecular basis of outer kinetochore assembly on CENP-T.
Petrovic A et al. Cell 2016; 167 (4):1028-1040.e15 Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Fang Y et al. Oncotarget 2016; 7 (48):79327-79341 Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.
Application : WB
Reactivity : Human
Zhou H et al. Nat Commun 2016; 7:10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : ICC/IF
Reactivity : Human
Petrovic A et al. Cell 2016; 167 (4):1028-1040 Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Application : WB
Reactivity : Human
Redli PM et al. J Cell Biol 2016; 215 (1):77-93 The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Application : ICC/IF
Reactivity : Human
Chung HJ et al. J Biol Chem 2016; (Epub) Phosphorylation of Astrin regulates its Kinetochore Function.
Application : ICC/IF
Reactivity : Human
DeLuca KF et al. Methods Mol Biol 2016; 1413:147-68 Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.
Application : ICC/IF
Reactivity : Human
Zhu L et al. J Biol Chem 2015; 290 (45):27053-66 Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
O'Connor A et al. Biol Open 2015; 5 (1):43413 Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint.
Application : ICC/IF
Reactivity : Human
Tauchman EC et al. Nat Commun 2015; 6:10036 Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
Kaczmarczyk A et al. PLoS One 2014; 9 (10):e106464 CENP-W plays a role in maintaining bipolar spindle structure.
Application : ICC/IF
Reactivity : Human
Espert A et al. J Cell Biol 2014; 206 (7):833-42 PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing.
Reactivity : Human
Hafner J et al. Nat Commun 2014; 5:4397 Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A.
Application : ICC/IF
Reactivity : Human
Huang LY et al. Cancer Chemother Pharmacol 2014; Inhibition of Hec1 as a novel approach for treatment of primary liver cancer.
Application : WB
Basilico F et al. Elife 2014; 3:e02978 The pseudo GTPase CENP-M drives human kinetochore assembly.
Application : WB, ICC/IF
Reactivity : Human
Matson DR et al. J Cell Biol 2014; 205 (4):541-554 CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.
Application : ICC/IF
Reactivity : Human
Eleni Petsalaki et al. Journal of Cell Biology 2014; 205 (3):339-56 Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Application : ICC/IF
Reactivity : Human
Huang LY et al. Mol Cancer Ther 2014; Activity of a Novel HEC1-Targeted Anticancer Compound Against Breast Cancer Cell Lines In Vitro and In Vivo.
Application : WB, ICC/IF
Reactivity : Human
Caldas GV et al. J Cell Biol 2013; 203 (6):957-69 KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Application : WB
Reactivity : Human
Buheitel J et al. EMBO J 2013; 32 (5):666-76 Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
Application : ICC/IF
Reactivity : Human
Beck J et al. Nat Cell Biol 2013; 15 (4):430-9 Ubiquitylation-dependent localization of PLK1 in mitosis.
Application : ICC/IF
Reactivity : Human
Lawo S et al. Nat Cell Biol 2012; 14 (11):1148-58 Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Application : WB
De Antoni A et al. J Cell Biol 2012; 199 (2):269-84 A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
Matson DR et al. Genes Dev 2012; 26 (6):542-7 A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Chan YW et al. J Cell Biol 2012; 196 (5):563-71 Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
Krenn V et al. J Cell Biol 2012; 196 (4):451-67 Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Wei R et al. Mol Biol Cell 2011; 22 (19):3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Application : ICC/IF
Reactivity : Kangaroo rat
Serio G et al. Proc Natl Acad Sci U S A 2011; 108 (42):17337-42 Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Application : ICC/IF
Reactivity : Human
Screpanti E et al. Curr Biol 2011; 21 (5):391-8 Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
Application : ICC/IF
Reactivity : Human
Tooley JG et al. Mol Biol Cell 2011; 22 (8):1217-26 The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
Application : WB
Reactivity : Human
Sundin LJ et al. Mol Biol Cell 2011; 22 (6):759-68 The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
Application : ICC/IF
Reactivity : Human
Barnhart MC et al. J Cell Biol 2011; 194 (2):229-43 HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Application : ICC/IF
Reactivity : Human
Santaguida S et al. EMBO J 2011; 30 (8):1508-19 Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
Application : ICC/IF
Reactivity : Human
Petrovic A et al. J Cell Biol 2010; 190 (5):835-52 The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
Application : WB
Zanet J et al. PLoS One 2010; 5 (12):e15701 A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Application : IHC-Fr
Reactivity : Human
Zeng K et al. J Cell Biol 2010; 191 (7):1315-32 Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Screpanti E et al. PLoS One 2010; 5 (7):e11603 A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Application : ICC/IF
Santaguida S et al. J Cell Biol 2010; 190 (1):73-87 Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Application : ICC/IF
Reactivity : Human
Wu G et al. Mol Biol Cell 2009; 20 (22):4686-95 Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Application : ICC/IF
Reactivity : Human
Chan YW et al. J Cell Biol 2009; 185 (5):859-74 Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Application : ICC/IF
Reactivity : Human
Wu G et al. Cancer Res 2008; 68 (20):2371765 Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Application : ICC/IF
Barr AR et al. Mol Cell Biol 2008; 28 (23):7199-211 MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
Application : ICC/IF
Reactivity : Human
Zhu H et al. J Cell Biol 2008; 183 (5):835-48 FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
Application : ICC/IF
Reactivity : Human
Miller SA et al. Curr Biol 2008; 18 (22):1785-91 Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Application : ICC/IF
Reactivity : Human
Jang CY et al. J Cell Biol 2008; 181 (2):255-67 DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
Application : ICC/IF
Reactivity : Human
Wong J et al. Mol Biol Cell 2008; 19 (5):2083-91 Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
Application : ICC/IF
Reactivity : Human
Montembault E et al. J Cell Biol 2007; 179 (4):601-9 PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Application : ICC/IF
Reactivity : Human
Wong J et al. J Cell Biol 2006; 173 (6):879-91 HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Application : ICC/IF
Reactivity : Human
Klein UR et al. Mol Biol Cell 2006; 17 (6):2547-58 Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations