APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-2μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX23393-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-YEKKATLIKSSEE, from the C Terminus of the protein sequence according to NP_006092.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Cellular Localization
Nucleus
Background
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
|
GTX23393 WB Image
WB analysis of HeLa lysate using GTX23393 Hec1 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX23393 IHC-P Image
IHC-P analysis of human liver using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX23393 IHC-P Image
IHC-P analysis of human adrenal gland using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
REFERENCE
There are currently no references for Hec1 antibody, C-term (GTX23393). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Hec1 antibody, C-term (GTX23393). Be the first to share your experience with this product.
