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Hec1 antibody, C-term

Anti-Hec1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX23393
Anti-Hec1 antibody, C-term used in Western Blot (WB). GTX23393
Anti-Hec1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX23393

Cat. No. GTX23393

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Hec1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX23393-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YEKKATLIKSSEE, from the C Terminus of the protein sequence according to NP_006092.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX23393

GTX23393 IHC-P Image

IHC-P analysis of human adrenal gland using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-Hec1 antibody, C-term used in Western Blot (WB). GTX23393

GTX23393 WB Image

WB analysis of HeLa lysate using GTX23393 Hec1 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Hec1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX23393

GTX23393 IHC-P Image

IHC-P analysis of human liver using GTX23393 Hec1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399