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Hec1 antibody [EPR5342]

Anti-Hec1 antibody [EPR5342] used in Western Blot (WB). GTX63082

Cat. No. GTX63082

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EPR5342

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Hec1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to residues near the C-terminus of Human HEC1.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody [EPR5342] used in Western Blot (WB). GTX63082

GTX63082 WB Image

WB analysis of (1) Jurkat, (2) HeLa, (3) NCCIT and (4) human spleen lysates (10 μg per lane) using HEC1 antibody [EPR5342] at a dilution of 1:10,000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399