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Hec1 antibody

Anti-Hec1 antibody used in Western Blot (WB). GTX110735

Cat No. GTX110735

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , H1299

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein  corresponding to human HEC1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950978

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus , Kinetochore

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody used in Western Blot (WB). GTX110735

GTX110735 WB Image

Sample
A: 30ug of A431 whole cell lysate (GTX27909)
B: 30ug of H1299 whole cell lysate
7.5% SDS PAGE
GTX110735 diluted at 1:1000

REFERENCE

REVIEW

Hec1 antibody Cat No. GTX110735

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2014. PMID: 24401611
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169