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Hec1 antibody

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

Cat No. GTX70016

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($379)
See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:750
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70016-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A Synthetic peptide within a.a. 30~80 around Ser55

Purification

Affinity purified using antigen

Conjugation

Unconjugated

RRID

AB_11171823

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

GTX70016 ICC/IF Image

Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379