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Hec1 antibody

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

Cat No. GTX70016

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($329)
See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:750
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70016-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A Synthetic peptide within a.a. 30~80 around Ser55

Purification

Affinity purified using antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ndc80, Kinetochore Complex Component , Hec , Hec1 , Hshec1 , Kntc2 , Tid3 , Hsndc80 , Ndc80

Cellular Localization

Nucleus , Kinetochore

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

GTX70016 ICC/IF Image

Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329