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Hec1 antibody

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

Cat. No. GTX70016

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:750
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70016-PEP)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A Synthetic peptide within a.a. 30~80 around Ser55

Purification

Affinity purified using antigen

Conjugation

Unconjugated

RRID

AB_11171823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70016

GTX70016 ICC/IF Image

Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399