Recommended Starting Dilutions:
For WB: Use at a dilution of 1:400
For ICC/IF: Use at a dilution of 1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
HeLa , Blocking peptide (GTX70014-PEP)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.816 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide (GTX70014-PEP) corresponding to the amino acids around Ser 76/77 conjugated to KLH
Affinity purified using antigen
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Ndc80, Kinetochore Complex Component , Hec , Hec1 , Hshec1 , Kntc2 , Tid3 , Hsndc80 , Ndc80
Nucleus , Kinetochore
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]