APPLICATION
Application Note
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:400
For ICC/IF: Use at a dilution of 1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
Calculated MW
Positive Control
HeLa , Blocking peptide (GTX70014-PEP)
PROPERTIES
Form
Liquid
Buffer
1X PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.816 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide (GTX70014-PEP) corresponding to the amino acids around Ser 76/77 conjugated to KLH
Purification
Affinity purified using antigen
Conjugation
Unconjugated
RRID
AB_11177349
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Cellular Localization
Nucleus
Background
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
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GTX70014 WB Image
Western Blot analysis of phospho and non-phospho Ser76/Ser77
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REFERENCE
There are currently no references for Hec1 (non-phospho Ser 76/77) antibody (GTX70014). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Hec1 (non-phospho Ser 76/77) antibody (GTX70014). Be the first to share your experience with this product.