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Hec1 (non-phospho Ser 76/77) antibody

Anti-Hec1 (non-phospho Ser 76/77) antibody used in Western Blot (WB). GTX70014

Cat No. GTX70014

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($329)
See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:400
For ICC/IF: Use at a dilution of 1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70014-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.816 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (GTX70014-PEP) corresponding to the amino acids around Ser 76/77 conjugated to KLH

Purification

Affinity purified using antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ndc80, Kinetochore Complex Component , Hec , Hec1 , Hshec1 , Kntc2 , Tid3 , Hsndc80 , Ndc80

Cellular Localization

Nucleus , Kinetochore

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hec1 (non-phospho Ser 76/77) antibody used in Western Blot (WB). GTX70014

GTX70014 WB Image

Western Blot analysis of phospho and non-phospho Ser76/Ser77

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329