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Hec1 (phospho Ser 165) antibody

Orthogonal
Anti-Hec1 (phospho Ser 165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70013
Anti-Hec1 (phospho Ser 165) antibody used in Western Blot (WB). GTX70013

Cat. No. GTX70013

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Kangaroo rat
Package
100 μl ($399)
See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70013-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.127 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide (GTX70013-PEP) corresponding to the amino acids around Ser 165 conjugated to KLH

Purification

Affinity purified using antigen

Conjugation

Unconjugated

RRID

AB_11174082

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 (phospho Ser 165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70013

GTX70013 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Anti-Hec1 (phospho Ser 165) antibody used in Western Blot (WB). GTX70013

GTX70013 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27764815

REFERENCE

Application Reference

Fang Y et al. Oncotarget 2016; 7 (48) : 79327-79341 Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.
Application : WB
Reactivity : Human
Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Application : ICC/IF
Reactivity : Kangaroo rat
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399