Hec1 (phospho Ser 165) antibody

Name: Hec1 (phospho Ser 165) antibody
Brand: GeneTex
SKU: GTX70013
Price: 379.00 USD
Rating: 5 (1 reviews)

Anti-Hec1 (phospho Ser 165) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70013

Cat No. GTX70013

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse, Rat, Kangaroo rat
Package	100 μl ($379)

See all Hec1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70013-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.127 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide (GTX70013-PEP) corresponding to the amino acids around Ser 165 conjugated to KLH

Purification

Affinity purified using antigen

Conjugation

Unconjugated

RRID

AB_11174082

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Gene ID: 10403 NDC80
UniProt: O14777 NDC80

Research Area

DATA IMAGES

GTX70013 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

REFERENCE

Application Reference

Fang Y et al. Oncotarget 2016; 7 (48) : 79327-79341 Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.

Application : WB

Reactivity : Human

Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.

Application : ICC/IF

Reactivity : Kangaroo rat

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