Hec1 (phospho Ser 55) antibody

Cat. No. GTX70017

Reference 16

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA
Reactivity	Human, Mouse, Rat, Kangaroo rat
Package	100 μl ($399)

See all Hec1 products

View product citations for antibody GTX70017 on CiteAb

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Human (100%)| Mouse (93%)| Bovine (86%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus , Kinetochore

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Database

Gene ID: 10403 NDC80
UniProt: O14777 NDC80

Research Area

DATA IMAGES

GTX70017 ICC/IF Image

Immunofluorescent staining of Hec1 phospho Ser55 in cells treated with Hec1 siRNA (bottom) or control siRNA (top)

GTX70017 WB Image

Purified 6his-Hec1 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-Hec1 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody

GTX70017 WB Image

GFP-tagged Hec1 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the Hec1 pSer55 antibody (diluted at 1:500)

GTX70017 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.