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Hec1 (phospho Ser 55) antibody

Anti-Hec1 (phospho Ser 55) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70017
Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017
Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017
Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017

Cat No. GTX70017

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA

Reactivity

Human, Mouse, Rat, Kangaroo rat
Package
100 μl ($329)

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide (GTX70017-PEP) corresponding to the amino acids around Ser 55 conjugated to KLH

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus , Kinetochore

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hec1 (phospho Ser 55) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70017

GTX70017 ICC/IF Image

Immunofluorescent staining of Hec1 phospho Ser55 in cells treated with Hec1 siRNA (bottom) or control siRNA (top)

Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017

GTX70017 WB Image

Purified 6his-Hec1 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-Hec1 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody

Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017

GTX70017 WB Image

GFP-tagged Hec1 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the Hec1 pSer55 antibody (diluted at 1:500)

Anti-Hec1 (phospho Ser 55) antibody used in Western Blot (WB). GTX70017

GTX70017 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329