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Heme Oxygenase 1 antibody [GT1334]

Orthogonal Overexpression
Anti-Heme Oxygenase 1 antibody [GT1334] used in Western Blot (WB). GTX633676
Anti-Heme Oxygenase 1 antibody [GT1334] used in Western Blot (WB). GTX633676

Cat. No. GTX633676

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1334

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Heme Oxygenase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa(500 μM CoCl2 treatment for 24 hr)

Product Note

This antibody is specific for human HMOX1 protein, and it does not cross react with human HMOX2 protein.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888359

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody [GT1334] used in Western Blot (WB). GTX633676

GTX633676 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [GT1334] used in Western Blot (WB). GTX633676

GTX633676 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations