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Heme Oxygenase 1 antibody [GT17811]

Orthogonal Overexpression
Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677
Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677
Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677
Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677
Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677

Cat. No. GTX633677

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT17811

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Heme Oxygenase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX633677, Detection: GTX101147
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa(500 μM CoCl2 treatment for 24 hr)

Product Note

This antibody is specific for human HMOX1 protein, and it does not cross react with human HMOX2 protein.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888360

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677

GTX633677 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677

GTX633677 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677

GTX633677 ICC/IF Image

Orthogonal

Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677

GTX633677 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000.

Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677

GTX633677 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations