APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture : GTX633677, Detection: GTX101147
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Not tested in other applications.
Calculated MW
Positive Control
HeLa(500 μM CoCl2 treatment for 24 hr)
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888360
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10
Cellular Localization
Microsome , Endoplasmic reticulum
Background
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX633677 ICC/IF Image
Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX633677 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000.
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GTX633677 ELISA Image
Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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REFERENCE
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REVIEW
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