APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX633677, Detection: GTX101147
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Not tested in other applications.
Calculated MW
Positive Control
HeLa(500 μM CoCl2 treatment for 24 hr)
Product Note
This antibody is specific for human HMOX1 protein, and it does not cross react with human HMOX2 protein.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888360
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10
Cellular Localization
Microsome , Endoplasmic reticulum
Background
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677](/templates/default/js/lightbox/images/loading.gif "Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677")
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GTX633677 ICC/IF Image
Heme Oxygenase 1 antibody [GT17811] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX633677 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:1000.
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GTX633677 ELISA Image
Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX633677 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT17811] (GTX633677) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Heme Oxygenase 1 antibody [GT17811] (GTX633677). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Heme Oxygenase 1 antibody [GT17811] (GTX633677). Be the first to share your experience with this product.
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677](/upload/website/prouct_img/small/GTX633677/GTX633677_42590_20180718_ICC_IF_CoCl2_w_23061202_299.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677](/upload/website/prouct_img/small/GTX633677/GTX633677_42590_20161013_WB_CoCl2_w_23061202_651.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677](/upload/website/prouct_img/small/GTX633677/GTX633677_42590_20190118_ELISA_PAIR_w_23061202_329.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677](/upload/website/prouct_img/small/GTX633677/GTX633677_42590_20230519_WB_multiple_B_23053001_670.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677](/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20180718_ICC_IF_CoCl2_w_23061202_299.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677](/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20161013_WB_CoCl2_w_23061202_651.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677](/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20190118_ELISA_PAIR_w_23061202_329.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in ELISA (ELISA). GTX633677")
![Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677](/upload/website/prouct_img/normal/GTX633677/GTX633677_42590_20230519_WB_multiple_B_23053001_670.webp "Anti-Heme Oxygenase 1 antibody [GT17811] used in Western Blot (WB). GTX633677")