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Heme Oxygenase 1 antibody [GT664]

Anti-Heme Oxygenase 1 antibody [GT664] used in ELISA (ELISA). GTX633693
Anti-Heme Oxygenase 1 antibody [GT664] used in Western Blot (WB). GTX633693
Anti-Heme Oxygenase 1 antibody [GT664] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633693

Cat No. GTX633693

Host

Mouse

Clonality

Monoclonal

Clone Name

GT664

Isotype

IgG1

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX633693, Detection: GTX101147

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , HeLa 500 μM CoCl2 treatment for 24 hr

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888368

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody [GT664] used in ELISA (ELISA). GTX633693

GTX633693 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633693 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [GT664] used in Western Blot (WB). GTX633693

GTX633693 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT664] (GTX633693) diluted at 1:1000.

Anti-Heme Oxygenase 1 antibody [GT664] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633693

GTX633693 ICC/IF Image

Heme Oxygenase 1 antibody [GT664] detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [GT664] (GTX633693) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations