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Heme Oxygenase 1 antibody [HL1780]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432
Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432

Cat. No. GTX637432

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1780

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Heme Oxygenase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody is specific for human Heme Oxygenase 1 protein, and it does not cross react with human Heme Oxygenase 2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Heme Oxygenase 1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome,Endoplasmic reticulum membrane

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432

GTX637432 WB Image

KO/KD

Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432

GTX637432 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432

GTX637432 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432

GTX637432 IHC-P Image

Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody [HL1780] used in Western Blot (WB). GTX637432

GTX637432 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432

GTX637432 IHC-P Image

Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody [HL1780] used in IHC (Paraffin sections) (IHC-P). GTX637432

GTX637432 IHC-P Image

Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations