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Heme Oxygenase 1 antibody

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

Cat. No. GTX101147

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX633677, Detection: GTX101147

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa,U87-MG,NIH-3T3

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950502

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147

GTX101147 ICC/IF Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal carcinoma .
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147

GTX101147 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 30621167

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Cells in 2019. PMID: 31366086

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the journal Cell Death Dis in 2017.PMID: 28055010

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

The data was published in the 2022 in Front Cell Neurosci. PMID: 35496911

REFERENCE

REVIEW

Heme Oxygenase 1 antibody Cat. No. GTX101147

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Normal spleen tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
Mariko Kogami et al. Oncol. Lett. 2023; 26:313
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169