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Heme Oxygenase 1 antibody

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147

Cat No. GTX101147

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, sELISA
Reactivity Human, Mouse, Rat, Monkey
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: GTX633677 , Detection: GTX101147

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , Raw264.7 , C2C12 , rat spleen , HeLa (500 μM CoCl2 treatment for 24 hr)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heme oxygenase 1,HMOX1D,HO-1,HSP32,bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal carcinoma .
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147

GTX101147 ICC/IF Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects HMOX1 protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147

GTX101147 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 µg/mL and GTX101147 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169