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Heme Oxygenase 1 antibody

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147
Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147
Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147
Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

Cat No. GTX101147

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX633677, Detection: GTX101147

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , Raw264.7 , C2C12 , rat spleen , HeLa (500 μM CoCl2 treatment for 24 hr)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950502

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101147

GTX101147 ICC/IF Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal carcinoma .
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in ELISA (ELISA). GTX101147

GTX101147 ELISA Image

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GTX633677 as capture antibody at concentration of 5 μg/mL and GTX101147 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Heme Oxygenase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101147

GTX101147 IHC-P Image

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Heme Oxygenase 1 antibody used in Western Blot (WB). GTX101147

GTX101147 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Heme Oxygenase 1 antibody Cat No. GTX101147

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2017.PMID: 28055010
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 30621167
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cells in 2019.PMID: 31366086
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Normal spleen tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf