GeneTex
United States (US)

Heme Oxygenase 1 antibody

GTX30748_IHC-P_18121410_920.jpg
Heme-Oxygenase-1-antibody_IHC_GTX30748-1_18121411_557.jpg
Heme-Oxygenase-1-antibody_WB_GTX30748-1_18121411_356.jpg
Heme-Oxygenase-1-antibody_IHC_GTX30748-2_18121411_604.jpg

Cat No. GTX30748

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Dog, Hamster, Guinea pig, Pig, Monkey
APPLICATION

Application Note

IHC: Use at an assay dependent dilution. WB: 1:2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

33 kDa. ( Note )

Positive Control

Recombinant Human and Rat HO-1 (Hsp32) Protein

Specificity/Sensitivity

May cross-react with HO-2 in western blot. It also recognizes a 50kD band found only in transformed cell lines but not in normal tissues.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 50% glycerol and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region

Purification

Protein A affinity purified
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heme Oxygenase 1, Ho1, Heox, 141250, hsp32, 3162, Heme Oxygenase1, P09601, Hmox1, Hmox, HO-1, bK286B10, HEOXG, HO 1

Background

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

Database

Research Area

DATA IMAGES
GTX30748_IHC-P_18121410_920.jpg

GTX30748 IHC-P Image

Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.

Heme-Oxygenase-1-antibody_IHC_GTX30748-1_18121411_557.jpg

GTX30748 IHC Image

Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.

Heme-Oxygenase-1-antibody_WB_GTX30748-1_18121411_356.jpg

GTX30748 WB Image

Western blot analysis of HO-1 pAb:
Lane 1: HO-1 Recombinant Protein
Lane 2: Human Liver Microsomes
Lane 3: Rat Liver Microsomes
Lane 4: Mouse Liver Microsomes
Lane 5: Canine Liver Microsomes

Heme-Oxygenase-1-antibody_IHC_GTX30748-2_18121411_604.jpg

GTX30748 IHC Image

Immunohistochemistry analysis of Alzheimer's diseased section stained with HO-1 (Hsp32) pAb.