IHC: Use at an assay dependent dilution. WB: 1:2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). Optimal dilutions/concentrations should be determined by the end user.
Recombinant Human and Rat HO-1 (Hsp32) Protein
May cross-react with HO-2 in western blot. It also recognizes a 50kD band found only in transformed cell lines but not in normal tissues.
Phosphate-buffered saline, pH 7.2, containing 50% glycerol and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region
Protein A affinity purified
From polyclonal serum
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Heme Oxygenase 1 , Heoxg , Heox , Hmox , Ho-1 , Ho1 , Hsp32 , Hmox1
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.