Application Note
IHC: Use at an assay dependent dilution. WB: 1:2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Recombinant Human and Rat HO-1 (Hsp32) Protein
Product Note
May cross-react with HO-2 in western blot. It also recognizes a 50kD band found only in transformed cell lines but not in normal tissues.
Form
Liquid
Buffer
PBS pH7.2, 50% Glycerol
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region
Purification
Protein A affinity purified
From polyclonal serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
heme oxygenase 1 , HEOXG , Heox , Hmox , Ho-1 , Ho1 , hsp32
Cellular Localization
Microsome , Endoplasmic reticulum membrane
Background
catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]
Database
Research Area