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Heme Oxygenase 2 antibody, Internal

Anti-Heme Oxygenase 2 antibody, Internal used in Western Blot (WB). GTX88386
Anti-Heme Oxygenase 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88386

Cat No. GTX88386

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Pancreas cancer shows increased staining of invading cancer cells.

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88386-PEP).

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DQAGSTLARETLED, from the internal region of the protein sequence according to NP_002125.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heme oxygenase 2 , HO-2

Cellular Localization

Microsome , Endoplasmic reticulum

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-Heme Oxygenase 2 antibody, Internal used in Western Blot (WB). GTX88386

GTX88386 WB Image

WB analysis of K562 cell lysate using GTX88386 Heme Oxygenase 2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Heme Oxygenase 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88386

GTX88386 IHC-P Image

IHC-P analysis of human pancreas: cancer (left), adjacent to cancer (right) using GTX88386 Heme Oxygenase 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf