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Hemoglobin alpha antibody [EGI-8]

Anti-Hemoglobin alpha antibody [EGI-8] used in IHC (Paraffin sections) (IHC-P). GTX04752
Anti-Hemoglobin alpha antibody [EGI-8] used in IHC (Paraffin sections) (IHC-P). GTX04752
Anti-Hemoglobin alpha antibody [EGI-8] used in Western Blot (WB). GTX04752

Cat. No. GTX04752

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EGI-8

Isotype

 IgG

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all Hemoglobin alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
FACS 1:50
IP 1:50
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150 mM NaCl, 50% glycerol, 0.4-0.5 mg/mL BSA

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Hemoglobin subunit alpha.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit alpha 1 , ECYT7 , HBA-T3 , HBH , METHBA

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin alpha antibody [EGI-8] used in IHC (Paraffin sections) (IHC-P). GTX04752

GTX04752 IHC-P Image

IHC-P analysis of human spleen tissue using GTX04752 Hemoglobin alpha antibody [EGI-8].

Anti-Hemoglobin alpha antibody [EGI-8] used in IHC (Paraffin sections) (IHC-P). GTX04752

GTX04752 IHC-P Image

IHC-P analysis of mouse embryo tissue using GTX04752 Hemoglobin alpha antibody [EGI-8].
Antigen retrieval : Heat mediated Tris-EDTA buffer (pH 8.0)

Anti-Hemoglobin alpha antibody [EGI-8] used in Western Blot (WB). GTX04752

GTX04752 WB Image

WB analysis of K562 whole cell lysate using GTX04752 Hemoglobin alpha antibody [EGI-8].

REFERENCE

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REVIEW

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Package List Price ($)
$ 469