GeneTex

  • Country / Location Selection

United States (US)

Hemoglobin alpha antibody

Anti-Hemoglobin alpha antibody used in Western Blot (WB). GTX04751
Anti-Hemoglobin alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04751

Cat. No. GTX04751

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Hemoglobin alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human HBA1; HBA2(Accession P69905), corresponding to amino acid residues D65-P115.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit alpha 1 , ECYT7 , HBA-T3 , HBH , METHBA

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin alpha antibody used in Western Blot (WB). GTX04751

GTX04751 WB Image

WB analysis of A2780 whole cell lysate using GTX04751 Hemoglobin alpha antibody. The lane on the left was treated with blocking peptide.

Anti-Hemoglobin alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04751

GTX04751 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04751 Hemoglobin alpha antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

There are currently no references for Hemoglobin alpha antibody (GTX04751). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Hemoglobin alpha antibody (GTX04751). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399