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Hemoglobin beta C antibody [15C2.C11.F2.G11]

Comparison Orthogonal
Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in Western Blot (WB). GTX04269
Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in ELISA (ELISA). GTX04269
Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in ELISA (ELISA). GTX04269
Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in Western Blot (WB). GTX04269

Cat. No. GTX04269

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 15C2.C11.F2.G11

Isotype

 IgG1

Application

 WB, ELISA, Lateral Flow

Reactivity

 Human
Package
100 μg ($669)
See all Hemoglobin beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ELISA 1:20000
Lateral Flow Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

This antibody detect the E6K mutant in the hemoglobin beta subunit and does not react with the HbA, HbS, HbF, or HbA-2 isoforms.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Purification

Protein A purified
From tissue cultue supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit beta , CD113t-C , ECYT6 , beta-globin

Cellular Localization

Extracellular region, Cytosol

Background

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in Western Blot (WB). GTX04269

GTX04269 WB Image

Orthogonal

WB analysis of various sample lysates using GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11].
Lane 1 : Prestained molecular weight marker
Lane 2 : HbA peptide conjugated to BSA
Lane 3 : HbA-2 peptide conjugated to BSA
Lane 4 : HbC peptide conjugated to BSA
Lane 5 : HbF peptide conjugated to BSA
Lane 6 : HbS peptide conjugated to BSA
Lane 7 : BSA alone
Loading : 50ng per lane
Dilution : 1μg/mL

Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in ELISA (ELISA). GTX04269

GTX04269 ELISA Image

Comparison

ELISA analysis of 0.1μg BSA-conjugated HbC peptide using GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11]. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Purple : GTX04269
Green : Hemoglobin polyclonal antibody

Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in ELISA (ELISA). GTX04269

GTX04269 ELISA Image

ELISA analysis of BSA-conjugated HbC peptide using serially diluted GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11].
Coating : 0.1 μg per well
Purple : GTX04269
Green : Hemoglobin polyclonal antibody

Anti-Hemoglobin beta C antibody [15C2.C11.F2.G11] used in Western Blot (WB). GTX04269

GTX04269 WB Image

WB analysis of various samples using GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11].
Lane 1 : Protein ladder
Lane 2 : HbA peptide conjugated to BSA
Lane 3 : HbA-2 peptide conjugated to BSA
Lane 4 : HbC peptide conjugated to BSA
Lane 5 : HbF peptide conjugated to BSA
Lane 6 : HbS peptide conjugated to BSA
Lane 7 : BSA
Loading : 50 ng
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 669
Currently in production