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Hemoglobin beta antibody [14G2.G11.F11]

Orthogonal
Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267
Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267
Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267
Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267
Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Lateral Flow (Lateral Flow). GTX04267

Cat. No. GTX04267

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14G2.G11.F11

Isotype

 IgG2a

Application

 WB, FACS, ELISA, Lateral Flow

Reactivity

 Human
Package
100 μg ($669)
See all Hemoglobin beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
FACS Assay dependent
ELISA 1:20000
Lateral Flow Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

This antibody cross reacts with HbA-2 but does not react the HbS, HbF (gamma), or HbC forms.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Purification

Protein A purified
From tissue cultue supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit beta , CD113t-C , ECYT6 , beta-globin

Cellular Localization

Extracellular region, Cytosol

Background

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267

GTX04267 WB Image

WB analysis of various samples using GTX04267 Hemoglobin beta antibody [14G2.G11.F11].
Lane 1 : Protein ladder
Lane 2 : Human HbA (0.1 μg)
Lane 3 : Human HbS (0.1 μg)
Lane 4 : Human heart whole cell lysate (2 μg)
Lane 5 : HeLa whole cell lysate (10 μg)
Dilution : 1 μg/mL

Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267

GTX04267 WB Image

WB analysis of various samples using GTX04267 Hemoglobin beta antibody [14G2.G11.F11].
Lane 1 : Protein Ladder
Lane 2 : HbA peptide conjugated to BSA
Lane 3 : HbA-2 peptide conjugated to BSA
Lane 4 : HbC peptide conjugated to BSA
Lane 5 : HbF peptide conjugated to BSA
Lane 6 : HbS peptide conjugated to BSA
Loading : 50 ng
Dilution : 1 μg/mL

Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267

GTX04267 WB Image

Orthogonal

WB analysis of various sample lysates using GTX04267 Hemoglobin beta antibody [14G2.G11.F11].
Lane 1 : Prestained molecular weight marker
Lane 2 : HbA peptide conjugated to BSA
Lane 3 : HbA-2 peptide conjugated to BSA
Lane 4 : HbC peptide conjugated to BSA
Lane 5 : HbF peptide conjugated to BSA
Lane 6 : HbS peptide conjugated to BSA
Loading : 50ng per lane
Dilution : 1μg/mL

Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Western Blot (WB). GTX04267

GTX04267 WB Image

Orthogonal

WB analysis of various sample lysates using GTX04267 Hemoglobin beta antibody [14G2.G11.F11].
Lane 1 : Prestained molecular weight marker
Lane 2 : Human HbA (0.1μg)
Lane 3 : Human HbS (0.1μg)
Lane 4 : Human heart whole cell lysate (2.0μg)
Lane 5 : HeLa cell lysate (10.0μg)
Dilution : 1μg/mL

Anti-Hemoglobin beta antibody [14G2.G11.F11] used in Lateral Flow (Lateral Flow). GTX04267

GTX04267 Lateral Flow Image

Orthogonal

Lateral flow analysis of recombinant HbA (left group) or HbS (right group) proteins using GTX04267 Hemoglobin beta antibody [14G2.G11.F11] and GTX04268 Hemoglobin beta S antibody [23E5.H6.G6.C1.H7.F7.G9.F6].
Antibody amount : GTX04267 Hemoglobin beta antibody 0.5μL at 250ug/mL and GTX04268 Hemoglobin beta S antibody 0.5μL at 1mg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 669
Currently in production