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Hemoglobin beta antibody

Orthogonal
Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412
Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412
Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412

Cat. No. GTX116412

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Hemoglobin beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

human plasma , Mouse Leukocyte , mouse leukocyte treated with CFA , rat spleen

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Hemoglobin beta Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167112

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit beta , CD113t-C , ECYT6 , beta-globin

Background

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412

GTX116412 WB Image

Orthogonal

Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500. (CFA: Complete Freunds adjuvant)

Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412

GTX116412 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500.

Anti-Hemoglobin beta antibody used in Western Blot (WB). GTX116412

GTX116412 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169